Divergence in chondrogenic potential between in vitro and in vivo of adipose- and synovial-stem cells from mouse and human

Abstract Background Somatic stem cell transplantation has been performed for cartilage injury, but the reparative mechanisms are still conflicting. The chondrogenic potential of cells thought as promising features therapy; however, correlation between their chondrogenesis in vitro and vivo remains undefined. purpose this study was to investigate intrinsic condition depends on types explore an indicator select useful regeneration. Methods two different derived from adipose tissue (ASCs) synovium (SSCs) mice humans assessed using bone morphogenic protein-2 (BMP2) transforming growth factor-β1 (TGFβ1). Their validated through into a mouse osteochondral defect model. Results All showed apparent under combination BMP2 TGFβ1 vitro, by formation proteoglycan- type 2 collagen (COL2)-rich tissues. However, our results vastly differed with those observed following single stimulation among species types; SSCs supplementation or TGFβ1, whereas ASCs human not TGFβ1. Human no obvious stimulation. Mouse hyaline-like which had less fibrous components (COL1/3) developed COL1/3+ tissues all treatments. Transcriptomic analysis TGFβ receptors ligands prior induction did indicate distinct reactivity BMP2. In transplanted site vivo, formed (proteoglycan+/COL2+/COL1−/COL3−) other mainly COL1/3-positive line Conclusion Optimal factors driving somatic intrinsically species. Among them, response may possibly represent fate when locally defects.